| Registro completo |
| Provedor de dados: |
J. Venom. Anim. Toxins incl. Trop. Dis.
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| País: |
Brazil
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| Título: |
Neutralization of the neuromuscular activity of bothropstoxin-i, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga)
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| Autores: |
Oshima-Franco,Y.
Alves,C. M. V.
Andréo Filho,N.
Gerenutti,M.
Cintra,A. C. O.
Leite,G. B.
Rodrigues-Simioni,L.
Silva,M. G.
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| Data: |
2005-12-01
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| Ano: |
2005
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| Palavras-chave: |
Alternative medicine
Phytotherapy
Plant extracts
Snakebite
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| Resumo: |
Numerous plants are used as snakebite antidotes in Brazilian folk medicine, including Casearia sylvestris Swartz, popularly known as guaçatonga. In this study, we examined the action of a hydroalcoholic extract from C. sylvestris on the neuromuscular blockade caused by bothropstoxin-I (BthTX-I), a myotoxin from Bothrops jararacussu venom, in mouse isolated phrenic nerve-diaphragm (PND) preparations. Aqueous (8 and 12 mg/ml, n=4 and 5, respectively) and hydroalcoholic (12 mg/ml, n=12) extracts of the leaves of C. sylvestris caused facilitation in PND preparations followed by partial neuromuscular blockade. BthTX-I (20 µg/ml, n=4) caused 50% paralysis after 65±15 min (mean ± S.E.M). Preincubation (30 min at 37° C) of BthTX-I (20 µg/ml, n=4) with a concentration of the hydroalcoholic extract (4 mg/ml) that had no neuromuscular activity, such as the control (n=5), prevented the neuromuscular blockade caused by the toxin. This protection may be mediated by compounds such as flavonoids and phenols identified by thin-layer chromatography and colorimetric assays.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400007
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| Editor: |
Centro de Estudos de Venenos e Animais Peçonhentos
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| Relação: |
10.1590/S1678-91992005000400007
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| Formato: |
text/html
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| Fonte: |
Journal of Venomous Animals and Toxins including Tropical Diseases v.11 n.4 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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